Biochem/physiol Actions

Reversible: no

Target IC₅₀: 0.8 and 2 µM against human and procine PDE II, respectively

Product does not compete with ATP.

Primary TargetPDE 2

Target K_i: 4 nM against adenosine deaminase

Cell permeable: yes

General description

The racemic mixture of a cell-permeable adenine derivative that acts as a PDE II-selective phosphodiesterase inhibitor (IC50 = 0.8 and 2 µM against human and procine PDE II, respectively) and adenosine deaminase (K_i = 4 nM), while exhibiting little or much reduced potency toward PDE I/II/IV isozymes (IC50 >100 µM) of both human and porcine species. EHNA at 10 µM concentration is also reported to effectively and reversibly block the differentiation and maintain the pluripotency of hES (human embryonic stem) cell line SA121 in feeder-free cultures without exogenously added FGF for up to 30 passages in a manner independent of its known ADA and PDE inhibitory activities.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Burton, P., et al. 2010. Biochem. J.in press.Lorbar, M., et al. 1999. J. Mol. Cell. Cardiol.31, 401.Verde, I., et al. 1999. Br. J. Pharmacol.127, 65.Reynolds, A.J., et al. 1996. Brain Res.798, 67.Mery, P.F., et al. 1995. Mol. Pharmacol.48, 121.Podzuweit, T., et al. 1995. Cell. Signal.7, 733.Bessodes, M., et al. 1982. Biochem. Pharmacol.31, 879.Bouchard, P., et al. 1981. Proc. Natl. Acad. Sci. USA78, 1033.

Packaging

10 mg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)